Axenic amastigote cultivation and in vitro development of Leishmania orientalis
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چکیده
منابع مشابه
Axenic cultivation and characterization of Leishmania mexicana amastigote-like forms.
A new method is described which has made possible the long-term axenic cultivation of Leishmania mexicana amastigote-like forms in Schneider's Drosophila medium supplemented with 20% (v/v) foetal calf serum. Unlike previous methods, it utilizes direct culture of parasites obtained from the lesions of infected animals rather than adaptation of promastigotes in vitro. Ultrastructural (possession ...
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Experimental infections with visceral Leishmania spp. are frequently performed referring to stationary parasite cultures that are comprised of a mixture of metacyclic and non-metacyclic parasites often with little regard to time of culture and metacyclic purification. This may lead to misleading or irreproducible experimental data. It is known that the maintenance of Leishmania spp. in vitro re...
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Corresponding Authors: Tanmoy Bera Department of Pharmaceutical Technology, Jadavpur University, Kolkata700032, India. E-mail: [email protected], Phone: +91 9831470041, Tele Fax: +91 3324146677. Abstract: Backgrounds and objectives: Leishmania donovani is one of the most common species responsible for visceral leishmaniasis (VL) in India, Bangladesh and Sudan. The pentavalent antimonials...
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The role of glycosphingolipids (GSLs) present in amastigote forms of Leishmania (Leishmania) amazonensis during infection of macrophages was analyzed, with particular emphasis on GSLs presenting the terminal Galpss1-3Galpa disaccharide. Macrophage invasion by L. (L.) amazonensis amastigotes was reduced by 37% when the disaccharide Galpss1-3Galp (1 mM) was added to the culture medium. The putati...
متن کاملThe structure of Leishmania major amastigote lipophosphoglycan.
Intracellular amastigotes of Leishmania major produce 6 x 10(4) copies/cell of a lipophosphoglycan (LPG) that is structurally distinct from the LPG produced by the extracellular promastigote form of L. major, Leishmania donovani, and Leishmania mexicana (reviewed by McConville, M. J. (1991) Cell Biol. Int. Rep. 15, 779-798). L. major amastigote LPG is composed of a lysoalkyl phosphatidylinosito...
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ژورنال
عنوان ژورنال: Parasitology Research
سال: 2019
ISSN: 0932-0113,1432-1955
DOI: 10.1007/s00436-019-06311-z